Test Details: Immunohistochemical testing is carried out on biopsy/resected tissue specimen. This testing helps to identify specific antigens (proteins) within tissue sections utilizing an antigen-specific antibody .Glypican-3 is a membrane and extracellular heparan sulfate glycoprotein that regulates signaling during embryogenesis. Glypican-3 is normally expressed in fetal tissue and trophoblasts. In adult tissue, the expression of glypican-3 is restricted to few tissue types, namely, gastric glands and renal tubules. Glypican-3 is also expressed in a wide range of epithelial and mesenchymal tumours including pulmonary squamous cell carcinoma and small cell carcinoma, hepatocellular carcinoma and hepatoblastoma, acinar carcinoma of the pancreas, neuroblastoma, Wilms’ tumour, yolk sac tumour and choriocarcinoma, liposarcoma, and rhabdomyosarcoma. It is used as part of a panel in differentiating hepatocellular carcinoma from benign liver lesions and metastatic neoplasms.