Test Details: Prothrombin gene mutation (or Factor II mutation or Prothrombin G20210A) is an inherited condition that increases predisposition to develop abnormal blood clots in the veins (deep vein thrombosis or DVT) and lungs (pulmonary embolism or PE). The test is indicated in following cases: 1. All patients with venous thrombosis, venous/pulmonary thromboembolism, thrombosis at different sites, coronary artery disease and/or stroke of unknown etiology. 2. Asymptomatic individuals with family history of venous thrombosis. 3. Individuals with family members known to have prothrombin mutation. 4. Thrombotic risk with oral contraceptives. 5. Women with recurrent pregnancy loss, unexplained severe pre-eclampsia, placenta abruption, fetal growth retardation, stillbirth or neural tube defects in offspring.