Test Details: MDR-TB is defined as TB that is resistant to the two first-line anti-TB drugs rifampicin and isoniazid. HAIN is the Line probe assay, DNA-based molecular test. The novel diagnostic test called MTBDRplus is a multiplex PCR based on DNA-strip technology that identifies genetic mutations in TB strains, making them resistant to isoniazid and rifampicin. MTBDRplus (HAIN first line) is used to detect Mycobacterium tuberculosis complex directly and isoniazid and rifampicin susceptibility to it, which is very helpful to diagnose MDR tuberculosis in a very short period of time. Resistance to rifampicin is a critical pre-requisite for identifying patients who can be put on second line anti-TB regimen.